The tomato rhizosphere microbiome influences root exudate composition via a systemic root–root signalling mechanism. This study showed that microbial colonization of one root can alter exudate composition in a distant root, naming this process as SIREM (systemically induced root exudation of metabolites). E.g., _{Bacillus subtilis} modulated the systemic exudation of the acyl sugars metabolite with insecticide function. This study provides evidence that microbiome-induced systemic exudation enhances soil conditioning and reveals new insights into microbiota-mediated root metabolism.